Hong-Bian Wei, Yan-Yan Gao, Ming-Sheng Zhang, Xiao-Hong Wang, Xue Li, Li Tian, Si-Jia Liu and Jian-Dong Liu
Psammosilene tunicoides. In this study, the stems, leaves and buds of P. tunicoides were used as explants, adding growth regulators (NAA, 2,4-D and 6-BA) in MS basic medium, and by adjusting the concentrations of growth regulators and the illumination intensity in culture room to induce callus, then the proliferated callus were used to extract anthocyanins. The results showed that the callus induction rate was the highest using buds as explants; it was about 1 to 2 times high of others (stems and leaves). NAA or 6-BA contributed to induce anthocyanins synthesis in the white callus. With the increase of 6-BA concentration, the anthocyanins content increased after slightly decreased, and the anthocyanins synthesis was promoted by increasing NAA concentration from 0.5 mg·L-1 to 1.5 mg·L-1 but decreased thereafter up to 2.5 mg·L-1. The combination screening of growth regulators indicated that the combination of 1.5 mg·L-1 NAA+0.5 mg·L-1 6-BA was the appropriate condition in the range of 0.5 mg·L-1 to 2.5 mg·L-1. The illumination intensity of 2000 lx was fit for anthocyanins formation in callus. The anthocyanins synthesis in callus was repressed when 2,4-D was added into medium. The results will provide reference for the pigments production in callus culture of P. tunicoides.
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