兽医科学与医学诊断杂志

Efficacy of Newly Developed Screening Test (D-Saft1) For Detection of Antibiotic Residues in Raw Milk Samples Collected from Khartoum State, Sudan

Limya Mohamed Warsma, Nazik Eltayeb Musa Mustafa, Suzan Mohammed Ibrahim and Ibtisam El Yas Mohamed El Zubeir

Aim: In this study a newly antibiotic screening test (D-SAFT1) was developed, it is based on activation of Lactobacillus casi (DSM 38124) embedded in dried milk particles.

Methods: For the preparation of the test mixture: powdered milk, lactose, standard bromocerol green indicator solution and 0.1 ml of 1.5 × 107 Lactobacillus casei MRS culture were added to each Universal bottles containing specific amount of antibiotic standard. The mixtures were frozen in a deep freezer at -200C for 24 hours. Then the bottles were lyophilized (–600C) and kept at 4-50C until used. Field samples of raw milk (200 cows, 50 camels and 50 goats) were collected from Khartoum State and examined for antibiotic residues using D-SAFT1 against two other approved methods that include Trisensor antibiotic test and the Modified One Plate Test.

Results: Tri sensor, Modified one plate test and the new detection method (D-SAFT1) revealed the same results that 80 (40%) of cow milk samples were positive to antibiotics residues, while all camel and goat milk samples were negative.

Conclusion: The antibiotic new detection method should be looked upon as an alternative screening method by encouraging its improvement, use and application as a field test, this especially because it can be prepared locally in addition to its low cost.