再生医学杂志

Adipose Derived Stem Cell Engraftment Improves Erectile Function in a Rat Model of Cavernosal Nerve Injury

James E. Anaissie1 , Amit G. Reddy1 , Bryant Song2 , Zahra Brabadi3 , Nora M. Haney4, Fatemeh Daneshimehr3 , Travis Chen2 , Kevin Swan2 , Kenneth J. DeLay5 , Laith Alzweri5 , Philip Kadowitz2 , Bysani Chandrasekar6,7 , Eckhard U. Alt3 , Wayne J.G. Hellstrom4 and Reza Izadpanah3

Adipose-derived-stem-cells (ADSC) have shown promise in treating
erectile dysfunction (ED). Here we investigated the effect of ADSC
engraftment in restoring erectile function (EF) following nerve injury
during radical prostatectomy. Sprague-Dawley rats (4 groups; n=8/
group) underwent: 1) laparotomy (Lap) and immediate closure
(Sham); 2) Lap with bilateral cavernosal nerve injury (BCNI) (Crush);
3) Lap with BCNI and intracavernosal injection (ICI) of GFP+-ADSC
at surgery (INJ-1); and 4) Lap with BCNI and ICI of GFP+-ADSC
twice (at surgery and after three weeks) (INJ-2). Six weeks postBCNI, EF was measured via intracorporal pressure (ICP) response
following cavernosal nerve stimulation at 2.5V, 5V, and 7.5V. Penile
and major pelvic ganglion (MPG) tissue were analyzed to detect
GFP+-ADSC by immunohistochemistry. Data showed a significant
decrease in EF in the Crush group compared to Sham at 5V and
7.5V (P<0.01). While EF was significantly improved in both INJ-1
and INJ-2 groups compared to the Crush group (5V and 7.5V;
P<0.01), it was comparable between INJ-1 and INJ-2 groups at
higher voltages. Interestingly, no GFP+-ADSCs were identified in
both penile and MPG tissues in all four groups 6 weeks post-BCNI.
These data indicate that a single intracavernosal administration of
ADSCs is sufficient to improve EF following nerve injury during
radical prostatectomy.