应用生物信息学与计算生物学杂志

In Silico Comparative Protein Analysis of Human Stem Cell factor (SCF)

Moein Dehbashi, Elahe Kamali and Sadeq Vallian

In Silico comparative protein analysis of Human Stem Cell factor (SCF)

Stem cell factor (SCF) is a critical protein with several key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. In the present study, the in silico analysis including phylogenetics, protein network analysis (interactome) and conservation assessment on SCF was performed using NCBIBLAST, MEGA6 software, STRING 10 database and ClustalX 2.1 software. Analysis of the shared SCF protein interactors in human (Homo sapiens), Gorilla (Gorilla gorilla) and Chimpanzee (Pan troglodytes) demonstrated the presence of partly either conserved proteins interactors or proteins with the same function. Moreover, the data showed that 152 amino acids of SCF were conserved among the examined primates. Together, the results approved the gene flow and genetics similarity of SCF among human, Gorilla and Chimpanzee. This may suggest that during evolution, SCF gene transferred partly intact either on the basis of protein sequence or function from the same ancestors to Gorilla, Chimpanzee and human.