Vadivukkarasi S , Manikandan M Pavithra K and Vasudevan
The protease enzyme from Cucurbita maxima peel was isolated and purified and its application in removing blood stain was studied. The optimum pH and temperature for this newly isolated protease was found to be 7.0 and 40°C respectively. The molecular weight of isolated protease was determined by SDS PAGE and it was found to be 31 KD. This new protease enzyme was applied to blood stained cloth to evaluate the stain removing capacity of the enzyme in the presence and absence of detergents. From the results, it is confirmed that the newly isolated protease removes stain completely with detergents and even in the absence of detergents. This study confirms the application of Cucurbita maxima peel protease in removing blood stains
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